The RTP® DNA/RNA Virus Mini Kit contains the pre-filled Extraction Tubes with lyophilized lysis components for viral DNA and RNA isolation from a diverse range of starting materials. After lysis the samples are transferred to a spin column-based procedure with a total preparation time of PCR/ RT-PCR templates in just 25 minutes.
- Simple „One-Step-Lysis“ in the pre-filled Extraction Tube with lyophilized lysis components (lysis buffer, Proteinase K, Carrier RNA); Extraction Tubes can be stored at room temperature
- Sensitive and accurate results at low viral titers
- For In Vitro Diagnostic Use (CE-IVD)*
- One kit for both DNA and RNA viral purification allowing simultaneous testing of both virus types
Starting Material Average Yield Preparation Time up to 200 μl serum, plasma, cell-free body fluids, cell culture supernatant
up to 400 µl rinse liquid from swabs
up to 10 mg tissue samples
depending on viral titer about 25 min
Using the RTP DNA/RNA Virus Mini Kit the sample is lysed in the pre-filled Extraction Tube containing lyophilized lysis reagents and carrier nucleic acid. The lysis reagents further stabilize the viral nucleic acids, inactivate RNases and DNases and enhance the selective DNA /RNA adsorption to the membrane of the Spin Filter. Before the viral nucleic acids are eluted, the membranes are efficiently washed in order to completely remove all PCR inhibitors.
*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.
1. Sensitive viral RNA isolation
200 µl of human plasma samples were spiked with Influenza A virus (1:10 dilution series) and viral RNA was isolated using the RTP DNA/RNA Virus Mini Kit (blue lines) and the Invisorb Spin Virus RNA Mini Kit (red lines). The figure shows the real-time amplification results, analyzed in triplicates. The black curve represents the negative control.
2. Reproducible recovery of viral DNA
Viral DNA was isolated from 10 different plasma samples (200 µl) spiked with 500 HBV copies using the RTP DNA/RNA Virus Mini Kit. The viral DNA (green and blue curves) was eluted in 100 µl and 2.5 µl (12.5 copies per PCR - theoretical amount) were analyzed in a HBV specific real-time PCR assay. The mean Ct standard deviation is 0.41. The red curve represents the positive control.
3. Sensitive detection of HBV virus
Virus DNA was isolated from 200 µl HBV infected patient serum sample using the RTP DNA/RNA Virus Mini Kit and eluted in 60 µl buffer. The samples were analyzed using the RoboGene Quantification Module for Hepatitis B virus (HBV) Genomes. The test was performed on an ABI PRISM 7000 SDS. 5 µl of eluted HBV sample DNA was used per RT-PCR run.
positive sample: HBV positive serum
sample 1: 3000 genome equivalents / ml
sample 2: 5,400,000 genome equivalents / ml
negative sample: HBV negative serum, obtained from healthy blood donors
The data show the amplification of positive samples (1, 2). The other curves represent the amplification of the ready -to use HBV DNA control (dilution series from 1,000,000 up to 10 molecules per well control strip).
Data kindly provided from Dr. T. Köhler, Roboscreen GmbH, Leipzig, Germany.
4. Sensitive detection of the Influenza virus from pharyngeal swab samples
A dilution series of Influenza A/H3N2 virus (strain: Moskau from107to 103) was prepared: The viral RNA was isolated using the RTP DNA/RNA Virus Mini Kit. 25 µl of the eluted viral RNA was used for the transcription to cDNA and 5 µl of the RT-PCR product was used for TaqMan-analysis for the positive detection of the viral infection.
Pharyngeal swab from patients with low concentration of the Influenza A virus was squeezed out in 4 ml medium. Viral RNA was isolated using the RTP DNA/RNA Virus Mini Kit from an aliquot of 200 µl of this medium. 25 µl was used for the transcription to cDNA and 5 µl of the RT-PCR product was used for TaqMan analysis for the positive detection of the viral infection.
Data kindly provided by Dr. Schweiger, Robert-Koch-Institute, Berlin, Germany
User manual download
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|Product||Package size||Catalogue No|
|RTP DNA/RNA Virus Mini Kit||50 purifications||1040100200|
|RTP DNA/RNA Virus Mini Kit||250 purifications||1040100300|