1. Digestion of plasmid DNA with restriction enzymes Eco RI or Hind III
Plasmid DNA (E.coli strain XL-1, vector pGEM) was purified from 2 ml of bacterial lysate using the Invisorb Plasmid HTS 96 Kit and subsequently digested with Eco RI or Hind III. 2 µl of the digested samples were loaded on an agarose gel.
2. Long read lengths in BigDye primer sequencing
The figure shows a BigDye primer sequence generated from vector pGEM purified with the Invisorb Plasmid HTS 96 Kit/ C on a centrifuge. The sequence was analyzed on an ABI PRISM 377 DNA sequencing system (Applied Biosystems) using a WTR gel. The pDNA consistently supports sequencing reactions that yield 900 bases of readable sequence.